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Metabolomics Facility

Introduction

Metabolomics refers to the measurement of small molecules, or metabolites, found in biological materials such as cells, tissues or biofluids. We provide targeted analyses and quantitation of specific metabolites encompassing a range of classes. We also advice on and provide analyses of experiments that use stable isotope tracer to probe the kinetics of metabolic pathways.  By assessing the condition- and/or time-dependent changes in particular metabolites in complex samples, we aim to gain insight into biological processes and establish new paths of research thus leading to novel, and more targeted, cancer therapies.

 

Instrument and software

The metabolomics facility is equipped with two high-resolution Thermo Scientific Q-Exactive mass spectrometers coupled with a Dionex UltiMate 3000 RSLC HPLC system and a Dionex ICS-6000 HPIC. These platforms are capable of separating and measuring small molecule metabolites with high accuracy and resolution. We use the Thermo Scientific Tracefinder software for data analyses and provide complete processing of the data we produce.

 

 instrument1

 instrument3

 

Sample submission and cost

The sample submission form and metabolomics checklist must be completed for all sample submissions. Samples are analysed according to previously agreed on dates and instrument suitability. For more information on cost, please see below

Sample submission form

Metabolomics checklist

Cost for external collaborators

 

Contact us

All collaborations are treated as academic collaborations and we will fully support a project from start to publication stage.

For initiation of new projects, please contact Dr Christian Frezza cf366@mrc-cu.cam.ac.uk

For details of sample preparation, submission and analyses, please contact the mass spec team mass-spec@mrc-cu.cam.ac.uk

 

Resources

Guidelines and Protocols for Metabolomics Analysis_Frezza Lab

Frezza lab home page

Reagents and consumables catalogue numbers