Probing cellular function and tissue architecture using new methods in imaging. CLICK HERE FOR A HIGH RESOLUTION IMAGE. A. Combining somatic cell genetics with fluorescence correlation spectroscopy for ‘in vivo biochemistry’ – molecular complexes formed by BRCA2 and their alteration after DNA damage (see Jeyasekharan et al Proc Nat Acad Sci USA(2010)). B. New FRET sensors for imaging biochemical reactions in DNA damage response pathways. C. Combining somatic cell genetics with fluorescence correlation spectroscopy for ‘in vivo biochemistry’ – reaction-diffusion mechanisms corral PLK1 kinase in the centrosome (see Mahen et al Proc Nat Acad Sci USA (2011)). D. Second harmonic generation (SHG) imaging microscopy, hyperdimensional imaging microscopy (HDIM) and H&E staining of histological sections. SHG and HDIM images are acquired with a non-labeled sample but provide high-contrast images.