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Workshop: Measuring Metabolic Engines and Fuels with the Seahorse XF Analyzer

When May 09, 2016 09:15 AM to
May 10, 2016 02:00 PM
Where Room 1.8, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, Cambridge.
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- Metabolism is the key to understanding cell function. In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes - 

Using the Seahorse XF Analyzer you can:

  • Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand.
  • Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
  • Identify fuel dependencies to uncover cancer cell vulnerabilities.
  • Explore how fuel preferences lead to cell fate decisions for differentiation and immune cell activation.

Presenters:

Sofia Viklstrom : Seahorse Bioscience, a part of Agilent Technologies

Edoardo Gaude : Christian Frezza Lab , MRC Cancer Unit

Priya Crosby : John O’Neill Lab, MRC LMB

Hiran Pirag : Mike Murphy Lab, MRC Mitochondrial Biology Unit

Stephen Burr : James Nathan Lab, CIMR

Simon Hoer : Patrick Maxwell Lab, CIMR


Agenda:

Monday, May 9, 2016

9:15 – 9:30  Participant Introduction

9:30 – 10:15   Seahorse Intro Presentation - Sofia Vikstrom

10:15 – 11:00  Software & Assay Optimization - Sofia Vikstrom

11:00 – 11:15  Coffee Break

11:15 – 12:00  “Phenotypic characterisation of   mtDNA mutation heteroplasmy shift” - Edoardo Gaude 

12:00 – 13:00  Lunch

13.00 – 13.30  “Circadian Biology” - Priya Crosby

13:30 – 14:00  “Using the Seahorse to explore mitochondrial function” - Hiran Pirag

14:00 -14:30   "Insights into the metabolic regulation   of HIFs by the oxoglutarate   dehydrogenase complex” - Stephen Burr

14:30 – 15:00   "Mitochondrial Kidney Disease” - Simon Hoer

15.00 – 15:30  Wet Lab Part I (Limited attendees)

Tuesday, May 10, 2016

9:00 – 14:00  Wet-Lab Part II (limited attendees!)

Please register by May 4, 2016 (indicating if you would like to join the wet lab session) with:

Karen Hébert: kah33@mrc-cu.cam.ac.uk

CONTACT: nick.howe@agilent.com


 

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