May 09, 2016 09:15 AM
May 10, 2016 02:00 PM
|Where||Room 1.8, Hutchison/MRC Research Centre, Cambridge Biomedical Campus, Cambridge.|
|Contact Name||Karen Hébert|
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- Metabolism is the key to understanding cell function. In living cells, most of the energy produced is derived from three fuel sources: glucose, glutamine, and fatty acids. Mitochondrial access to these fuels impacts a wide variety of biological processes -
Using the Seahorse XF Analyzer you can:
- Determine whether/how cells can adjust fuel oxidation to match nutrient availability while meeting energy demand.
- Distinguish metabolic adaptations due to genetic changes vs. those that take place due to nutrient deprivation.
- Identify fuel dependencies to uncover cancer cell vulnerabilities.
- Explore how fuel preferences lead to cell fate decisions for differentiation and immune cell activation.
Sofia Viklstrom : Seahorse Bioscience, a part of Agilent Technologies
Edoardo Gaude : Christian Frezza Lab , MRC Cancer Unit
Priya Crosby : John O’Neill Lab, MRC LMB
Hiran Pirag : Mike Murphy Lab, MRC Mitochondrial Biology Unit
Stephen Burr : James Nathan Lab, CIMR
Simon Hoer : Patrick Maxwell Lab, CIMR
Monday, May 9, 2016
9:15 – 9:30 Participant Introduction
9:30 – 10:15 Seahorse Intro Presentation - Sofia Vikstrom
10:15 – 11:00 Software & Assay Optimization - Sofia Vikstrom
11:00 – 11:15 Coffee Break
11:15 – 12:00 “Phenotypic characterisation of mtDNA mutation heteroplasmy shift” - Edoardo Gaude
12:00 – 13:00 Lunch
13.00 – 13.30 “Circadian Biology” - Priya Crosby
13:30 – 14:00 “Using the Seahorse to explore mitochondrial function” - Hiran Pirag
14:00 -14:30 "Insights into the metabolic regulation of HIFs by the oxoglutarate dehydrogenase complex” - Stephen Burr
14:30 – 15:00 "Mitochondrial Kidney Disease” - Simon Hoer
15.00 – 15:30 Wet Lab Part I (Limited attendees)
Tuesday, May 10, 20169:00 – 14:00 Wet-Lab Part II (limited attendees!)
Please register by May 4, 2016 (indicating if you would like to join the wet lab session) with:
Karen Hébert: firstname.lastname@example.org