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sponge22

 
Sponge

Cytosponge device shown contained in its gelatin sheath and when expanded.

rf1

 
rf group1

 
pjgrp2

 
pjgrp

 
PJfig2

Lineage tracing reveals how progenitors maintain oesophageal epithelium (basal layer is shown, blue progenitor cell, red differentiating cell)

PJ1

3D imaging of a 3 cell clone derived from individual epidermal progenitor cell genetically labelled 3 weeks previously, blue DNA stain, yellow EYFP.

papilla detail

Normal oesophageal epithelium stained for cytokeratin 14 (red) and DAPI (Blue).

p jones

 
p jones

 
oeso white

 
oeso WGA

 
nr cells2

 
js13a

Determining the interactions between murine melanoma cells and stroma. The addition of cancer-associated fibroblasts (red) to podoplanin-expressing melanoma cells (green) cultured within a 3-dimensional matrix stimulates invasive properties (lower panel) when compared with normal fibroblasts (red, top panel). After 2 days of culture with normal fibroblasts, podoplanin-expressing tumour cells retain their spheroid morphology. However, when alone or in the presence of cancer-associated fibroblasts, podoplanin- expressing cells outgrow the original spheroid structure and numerous podoplanin positive extensions can be seen penetrating the matrix.

nr cells2

 
imaging

 
nr cells

 
jsFig2

 
js15

 
MRC CU Hutchison map 2014s

 
helix 2

 
helix 5

 
helix 4

 
MRC CU Hutchison map 2014

 
helix 3

 
helix 1

 
helix

 
FrezzaFig1th

 
FrezzaFig1

 
dna av1

 
cpm2

Understanding the mechanisms of lung tumour evolution in vivo: KrasG12D-driven lung tumour showing up-regulation of p19ARF during low-to-high grade transition (from Junttila, et al. (2010) Nature 468, 567-571).

Fig 2 PJ

 
chro av2

 
CBMT image

 
brca2 av4

 
Bird lieberman 4a

CLICK HERE FOR A HIGH RESOLUTION IMAGE. Images taken with an endoscope: (A left picture) White light image, (A middle picture) Imaging fluorescence at 490-560 nm pre application of WGA, (A right picture). Imaging fluorescence at 490-560 nm post application of WGA-Alexa Fluor™ 488. The areas of low WGA binding appear purple. The dashed white line is placed longitudinally along the posterior wall of the esophagus to facilitate orientation between different images and the numbers 7, 8, and 9 refer to the y-coordinates on the reference grid in (B). Grid displaying the pathological diagnostic map (color coded with dark color representing a worsening grade of dysplasia) of each block made from the resection specimen. This same grid can be compared with the endoscopic and IVIS fluorescence images in A (right picture) and D. The dashed line represents the longitudinal axis along the posterior wall of the esophagus.The same specimen after being opened longitudinally along the anterior border of the esophagus is displayed with the overlying grid from B. WGA fluorescence signal from the esophageal specimen taken with the IVIS 200 camera. The pink arrow marks an area of artefact from exposed submucosal tissue and the blue arrow indicates the site of a previous endoscopic mucosal resection (surrounded by grey box).

Barretts

3D reconstructions of a Barrett’s crypt stained for cytokeratin 8/18 (green) and DAPI (blue).

brac2 av3

 
b&w cells sm

 
b&w cells sm

 
AV3

Structure of a complex (top) between RAD51 (magenta/blue) and BRCA2 (green) predicts their possible functions in DNA recombination (bottom, from Venkitaraman (2002) Cell 108, 171-182).

AV2

AURORA-A over-expression, which occurs in 30-50% of common cancers, over-rides the mitotic spindle assembly checkpoint mediated by MAD2, allowing anaphase entry with lagging chromosomes. Aurora-A over-expressing cells (right) or control cells (left) in prometaphase (top) or anaphase (bottom). DNA is stained red and MAD2, green.

AV1

Chromosomal aberrations (top) occur when pathways for DNA recombination (bottom) are defective. Top panel from Patel, et al. (1998) Mol Cell 1, 347-357. Bottom panel from Venkitaraman (2003) N Engl J Med 348, 1917-1919.

4gene signature

 
JS updated image 1

 
JS updated image 2

 
JS updated image 3

 
JS updated image 4

 
Birdliebermancropped.png

CLICK HERE FOR A HIGH RESOLUTION IMAGE. Images taken with an endoscope: (A left picture) White light image, (A middle picture) Imaging fluorescence at 490-560 nm pre application of WGA, (A right picture). Imaging fluorescence at 490-560 nm post application of WGA-Alexa Fluor™ 488. The areas of low WGA binding appear purple. The dashed white line is placed longitudinally along the posterior wall of the esophagus to facilitate orientation between different images and the numbers 7, 8, and 9 refer to the y-coordinates on the reference grid in (B). Grid displaying the pathological diagnostic map (color coded with dark color representing a worsening grade of dysplasia) of each block made from the resection specimen. This same grid can be compared with the endoscopic and IVIS fluorescence images in A (right picture) and D. The dashed line represents the longitudinal axis along the posterior wall of the esophagus.The same specimen after being opened longitudinally along the anterior border of the esophagus is displayed with the overlying grid from B. WGA fluorescence signal from the esophageal specimen taken with the IVIS 200 camera. The pink arrow marks an area of artefact from exposed submucosal tissue and the blue arrow indicates the site of a previous endoscopic mucosal resection (surrounded by grey box).